京尼平
丝素
材料科学
壳聚糖
组织工程
纳米纤维
丝胶
丝绸
生物医学工程
高分子科学
复合材料
化学工程
医学
工程类
作者
Kuihua Zhang,Yongfang Qian,Hongsheng Wang,Li Fan,Chen Huang,Anlin Yin,Xiumei Mo
摘要
Abstract To improve water‐resistant ability and mechanical properties of silk fibroin (SF)/hydroxybutyl chitosan (HBC) nanofibrous scaffolds for tissue‐engineering applications, genipin, glutaraldehyde (GTA), and ethanol were used to crosslink electrospun nanofibers, respectively. The mechanical properties of nanofibrous scaffolds were obviously improved after 24 h of crosslinking with genipin and were superior to those crosslinked with GTA and ethanol for 24 h. SEM indicated that crosslinked nanofibers with genipin and GTA vapor had good water‐resistant ability. Characterization of the microstructure (porosity and pore structure) demonstrated crosslinked nanofibrous scaffolds with genipin and GTA vapor had lager porosities and mean diameters than those with ethanol. Characterization of FTIR‐ATR and 13 C NMR clarified both genipin and GTA acted as crosslinking agents for SF and HBC. Furthermore, genipin could induce SF conformation from random coil or α‐helix to β‐sheet. Although GTA could also successfully crosslink SF/HBC nanofibrous scaffolds, in long run, genipin maybe a better method due to lower cytotoxicity than GTA. Cell viability studies and wound‐healing test in rats clarified that the genipin‐crosslinked SF/HBC nanofibrous scaffolds had a good biocompatibility both in vitro and in vivo . These results suggested that genipin‐crosslinked SF/HBC nanofibrous scaffolds might be potential candidates for wound dressing and tissue‐engineering scaffolds. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
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