序列(生物学)
突变
生物
人类免疫缺陷病毒(HIV)
打开阅读框
计算生物学
核糖核酸
细胞生物学
心理压抑
功能(生物学)
遗传学
肽序列
病毒学
突变
基因
基因表达
作者
E T Dayton,Douglas M. Powell,Andrew I. Dayton
出处
期刊:Science
[American Association for the Advancement of Science]
日期:1989-12-22
卷期号:246 (4937): 1625-1629
被引量:152
标识
DOI:10.1126/science.2688093
摘要
Expression of high levels of the structural proteins of the human immunodeficiency virus type 1 (HIV-1) requires the presence of the protein encoded by the rev open reading frame (Rev) and its associated target sequence CAR (cis anti-repression sequence) which is present in the env region of viral RNA. Extensive mutagenesis demonstrated that CAR has a complex secondary structure consisting of a central stem and five stem/loops. Disruption of any of these structures severely impaired the Rev response, but many of the stem/loops contain material that was unnecessary for Rev regulation and must be retained in these structures to avoid disturbing adjacent structures critical for CAR function. Probably no more than two of the described structural components are involved in sequence-specific recognition by regulatory proteins.
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