生物
基因组
全基因组测序
遗传学
参考基因组
DNA测序
基因组学
计算生物学
基因
基因组进化
基因组大小
顺序装配
结构变异
癌症基因组测序
进化生物学
作者
Robert A. Martienssen,Pablo D. Rabinowicz,A. O'Shaughnessy,W. Richard McCombie
标识
DOI:10.1016/j.pbi.2004.01.010
摘要
Sequencing of complex genomes can be accomplished by enriching shotgun libraries for genes. In maize, gene-enrichment by copy-number normalization (high C(0)t) and methylation filtration (MF) have been used to generate up to two-fold coverage of the gene-space with less than 1 million sequencing reads. Simulations using sequenced bacterial artificial chromosome (BAC) clones predict that 5x coverage of gene-rich regions, accompanied by less than 1x coverage of subclones from BAC contigs, will generate high-quality mapped sequence that meets the needs of geneticists while accommodating unusually high levels of structural polymorphism. By sequencing several inbred strains, we propose a strategy for capturing this polymorphism to investigate hybrid vigor or heterosis.
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