Topology‐Selective Chromatography Reveals Plasmid Supercoiling Shifts during Fermentation and Allows Rapid and Efficient Preparation of Topoisomers

Recognizing pDNA supercoils: Differently supercoiled species of the same plasmid DNA can be separated by topology-selective chromatography. Two-dimensional HPLC proved that the supercoiling changes during fermentation. Thus, a new quality criterium might help to optimize the effectivity of future genetic drugs and vaccines.