β-2微球蛋白
主要组织相容性复合体
MHC限制
抗原
MHC I级
表位
肽
分子生物学
化学
T细胞受体
融合蛋白
生物
重组DNA
免疫系统
细胞生物学
T细胞
生物化学
免疫学
基因
作者
Tim F. Greten,Firouzeh Korangy,Gunnar Neumann,Heiner Wedemeyer,Karola Schlote,Astrid Heller,Stephan Scheffer,Drew M. Pardoll,Annette I. Garbe,Jonathan P. Schneck,Michael P. Manns
标识
DOI:10.1016/s0022-1759(02)00346-0
摘要
Recombinant soluble MHC molecules are widely used for visualization, activation and inhibition of antigen-specific immune responses. Using a genetic approach, we have generated two novel peptide-beta2-microglobulin-MHC constructs. We have linked the MHC molecule with the peptide of interest, without limiting the recognition by the cognate TCR. This molecule can also be joined with the IgG heavy chain resulting in a dimeric MHC-Ig fusion protein. These molecules bind antigen-specific T cells with high specificity and sensitivity, therefore, providing a valuable tool for detection as well as enrichment of antigen-specific T cells.
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