Oxidation of Lipids. I. Quantitative Determination of the Oxidation of Methyl Linoleate and Methyl Linolenate

Abstract The extent of oxidation of methyl linoleate and methyl linolenate has been measured quantitatively by several methods such as oxygen uptake, substrate disappearance, and formation of conjugated diene, TBA-reactive material and of hydrocarbon gas. In the early stage of oxidation of methyl linoleate, oxygen uptake, substrate disappearance, peroxide and conjugated diene formation were all quantitative and their results agreed well each other. Little TBA-reactive material and hydrocarbons were observed. In the oxidation of methyl linolenate, however, no product was formed quantitatively, and the extent of oxidation estimated decreased in the order, oxygen uptake>substrate disappearance>peroxide>conjugated diene>TBA-reactive material.