共域化
荧光显微镜
显微镜
计算生物学
功能(生物学)
计算机科学
细胞内
生物
生物物理学
细胞生物学
荧光
病理
物理
医学
光学
作者
Kenneth W. Dunn,Malgorzata M. Kamocka,John H. McDonald
出处
期刊:American Journal of Physiology-cell Physiology
[American Physiological Society]
日期:2011-04-01
卷期号:300 (4): C723-C742
被引量:1608
标识
DOI:10.1152/ajpcell.00462.2010
摘要
Fluorescence microscopy is one of the most powerful tools for elucidating the cellular functions of proteins and other molecules. In many cases, the function of a molecule can be inferred from its association with specific intracellular compartments or molecular complexes, which is typically determined by comparing the distribution of a fluorescently labeled version of the molecule with that of a second, complementarily labeled probe. Although arguably the most common application of fluorescence microscopy in biomedical research, studies evaluating the “colocalization” of two probes are seldom quantified, despite a diversity of image analysis tools that have been specifically developed for that purpose. Here we provide a guide to analyzing colocalization in cell biology studies, emphasizing practical application of quantitative tools that are now widely available in commercial and free image analysis software.
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