Objective To observe the bactericidal efficacy of alcohol-based hand-disinfectant to clinical isolated nosocomial infection pathogens.Method Suspension quantitative bactericidal test was used in laboratory to test the bactericidal efficacy of the disinfectent to nosocomial infection pathogens compared with standard bacterial.Result The alcohol-based hand-disinfectant content 5 000 mg/L gluconate chlorhexidine and 75% alcohol.The average killing rates for Staphylococcus aureus clinical isolated by the disinfectant diluted to 6.25% was 99.83%.The average killing rates for Pseudomonas aeruginosa,Acinetobacter baumannii,Enterococcus faecalis and Candida albicans all reached over 99.9%.At the same condition,the average killing rates to the standard strain were 99.94%-100%.Conclusion The resistance to gluconate chlorhexidine and alcohol of the clinical isolated pathogens were higher than the standard strain.