Primary Study on Lactobacillus casei Starter Powder with High Activity

The conditions of high concentration cultivation and the drying methods of Lactobacillus casei were studied as starter powder in this paper. Acidity of the culture medium was not adjusted initially, the pH was kept at the range of 6.3 to 6.6 by adding the neutralizer of saturated Ca(OH)2, and the population of Lactobacillus casei approached 10~10/mL without fed batch cultivation. The concentration of Lactobacillus casei was suspended in the liquid of 100_0 NFMS and 2.70_0 glycerin before freeze-drying and the population of Lactobacillus casei reached 9.0×10~10/g.