Response surface methodology optimizing derivatization condition of taurolidine

Objective:On the base of the single factor investigation,response surface mathodology was applied to optimize the derivatization condition of taurolidine.Methods:Different derivatization reagent concentrations,reaction time and reaction temperature were taken as factor.The amount of the derivative products(expressed by peak area)was taken as response factor.Results:The optimizing derivatization condition of taurolidine was as follows:the reaction temperature(100 ℃),the derivatization reagent concentration(20 g·L-1),the reaction time(88 min).Conclusion:The method is accurate,reliable and can be applied to derivatize taurodiline.