日本使徒
细胞凋亡
生物
小RNA
荧光素酶
海参
非翻译区
分子生物学
互补DNA
报告基因
转染
细胞培养
细胞生物学
信使核糖核酸
基因表达
基因
生物化学
遗传学
生态学
作者
Ming Guo,Minjie Gui,Xi Xu,Xuemei Duan,Xuelin Zhao,Weiwei Zhang,Yina Shao,Bei Wang,Jing Diao,Chenghua Li
标识
DOI:10.1016/j.ijbiomac.2021.08.097
摘要
MicroRNAs (miRNAs) are closely related to the occurrence, development, and immune response of diseases. BCL2-associated athanogene 2 (BAG2) is a member of the BAG family that functions in diverse cellular processes, including cell death, differentiation, and cell division. In this study, we cloned the cDNA full-length of sea cucumber (Apostichopus japonicus) BAG2 (AjBAG2) and confirmed it is an anti-apoptotic protein in vitro and in vivo during Vibrio splendidus infection. Moreover, we identified a perfect complementarity between miR-375 and the 3'-untranslated region (UTR) sequence of AjBAG2. The miR-375 expression decreased the luciferase activity dose-dependently when co-transfected with the AjBAG2 3'-UTR-luciferase reporter containing the miR-375 target site in epithelioma papulosum cyprini (EPC) cells. This inhibition was partially recovered by a miR-375 specific inhibitor. The mRNA and protein levels of AjBAG2 were opposite to that of coelomocytes in challenged sea cucumber when treated with miR-375 mimics or inhibitors. Additionally, miR-375 expression induced coelomocytes apoptosis and blocked the anti-apoptotic activity of AjBAG2. Our data demonstrated that AjBAG2 is an anti-apoptotic protein during V. splendidus infection and this function can be inhibited by miR-375 in sea cucumbers.
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