诱导多能干细胞
细胞生物学
胚胎干细胞
化学
胚状体
干细胞
再生医学
细胞
定向微分
细胞培养
生物
细胞分化
电池类型
作者
Tadashi Takaki,Yoshinori Yoshida
出处
期刊:Methods of Molecular Biology
日期:2021-01-01
卷期号:2320: 101-110
标识
DOI:10.1007/978-1-0716-1484-6_11
摘要
FluoVolt, a membrane potential dye, has been used to depict the action potentials of cardiomyocytes derived from human-induced pluripotent stem cells (hiPSC-CMs) in order to classify the cardiac cell subtype, evaluate long QT syndrome, and conduct cardiotoxic drug-responsive tests. To apply FluoVolt, users must prepare the hiPSC-CMs, assess the dye loadings, and apply the excitation light. Here we describe the steps to measure action potentials from single hiPSC-CMs and hiPSC-CM monolayers using this dye.
科研通智能强力驱动
Strongly Powered by AbleSci AI