Termicin silencing enhances the toxicity of Serratia marcescens Bizio (SM1) to Odontotermes formosanus (Shiraki)

Termites are often exposed to a variety of pathogens during their life cycle, which has led to the development of an innate immune system to resist these pathogens. Antimicrobial peptides (AMPs) play a crucial role in the innate immune system in insects. However, clear information on AMPs in termites has not been obtained. Therefore, exploring the function of AMPs in the subterranean termite Odontotermes formosanus (Shiraki) can lead to the development of novel termite control strategies that integrate RNA interference (RNAi) and pathogens. Here we first obtained two Oftermicins from O. formosanus and observed that the expression of these Oftermicin genes was significantly upregulated at the mRNA level after treatment with lipopolysaccharide (LPS) or Serratia marcescens Bizio (SM1). Interestingly, the expression of these Oftermicins increased not only in the donor termites but also in the recipient termites through transmission experiments. Bioassay experiments showed that the mortality of O. formosanus treated with SM1 after RNAi was significantly higher than that of other groups. In summary, dsOftermicins are important immunosuppressants for termite control and Oftermicins are optimal targets for termite control based on the combined use of RNAi and pathogens.