脂肪生成
脂肪细胞
IC50型
吡咯里嗪
化学
植物疗法
生物
生物活性
生物化学
药理学
脂肪组织
体外
立体化学
医学
病理
替代医学
作者
Shota Uesugi,Mayuka Hakozaki,Yoshihiro Kanno,Akio Shiraishi,Mizuki Suzuki,Ken‐ichi Kimura,Yoshihito Shiono,Akira Yano
出处
期刊:Fitoterapia
[Elsevier]
日期:2022-03-01
卷期号:157: 105130-105130
被引量:1
标识
DOI:10.1016/j.fitote.2022.105130
摘要
Petasites japonicus is one of the most popular edible wild plants in Japan. Many biological effects of P. japonicus have been reported, including anti-allergy, anti-inflammation, and anticancer effects. Although its anti-obesity effect has been reported in several studies, the most important component responsible for this activity has not been fully elucidated. On screening the components that suppress adipocyte differentiation in 3T3-F442A cells, we found that the extract of the flower buds of P. japonicus has anti-adipogenic effect. Among the known major components of P. japonicus, petasin exhibited a potent anti-adipogenic effect at an IC50 value of 0.95 μM. Quantitative analysis revealed that the active component responsible for most of the anti-adipogenic effects of P. japonicus extract is petasin. Petasin suppressed the expression of markers of mature adipocytes (PPARγ, C/EBPα, and aP2). However, as isopetasin and petasol, analogs of petasin, did not exhibit these effects, it indicates that a double bond at the C11-C12 position and an angeloyl ester moiety were essential for the activity. Petasin affected the late stage of adipocyte differentiation and inhibited the expression of lipid synthesis factors (ACC1, FAS, and SCD1). Additionally, it was revealed that petasin could be efficiently extracted using hexane with minimal amount of pyrrolizidine alkaloids, the toxic components. These findings indicate that P. japonicus extract containing petasin could be a promising food material for the prevention of obesity.
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