Skin regenerative potential of polydeoxyribonucleotide isolated from Saussureainvolucrata

Saussurea involucrata, commonly known as snow lotus, is a rare medicinal plant that is traditionally used in several countries owing to its therapeutic properties. Snow lotus extracts have been shown to exert anti-inflammatory effects and reduce reactive oxygen species levels. Although various bioactive compounds have been identified in snow lotus, the biological activity and underlying mechanisms of DNA isolated from this plant remain unexplored. This study aimed to investigate the skin-regenerative properties of polydeoxyribonucleotide (PDRN) isolated from snow lotus. PDRN was extracted and purified from dried flowers of S. involucrata. Water-soluble tetrazolium salt 1, wound healing, and enzyme-linked immunosorbent assays were used to evaluate the effects of snow lotus PDRN on cell proliferation, cell migration, and collagen synthesis, respectively. We also measured matrix metalloproteinase 1 (MMP1) mRNA expression after snow lotus PDRN treatment. Snow lotus-derived PDRN was non-cytotoxic to human skin cells and significantly promoted cell proliferation and migration. Additionally, it enhanced collagen synthesis by suppressing the expression of MMP1. These findings demonstrate that snow lotus PDRN may be a promising anti-aging agent and may serve as a valuable ingredient in cosmeceutical formulations.