AllTheBacteria - all bacterial genomes assembled, available and searchable

Abstract The bacterial sequence data publicly available at the global DNA archives is a vast source of information on the evolution of bacteria and their mobile elements. However, most of it is either unassembled or inconsistently assembled and QC-ed. This makes it unsuitable for large-scale analyses, and inaccessible for most researchers to use. In 2021 Blackwell et al therefore released a uniformly assembled set of 661,405 genomes, consisting of all publicly available whole genome sequenced bacterial isolate data as of November 2018, along with various search indexes. In this study we extend that dataset up to August 2024, more than tripling the number of genomes. We also expand the scope, as we begin a global collaborative project to generate annotations for different species as desired by different research communities. In this study we describe the project as of release 2024-08, comprising 2,440,377 assemblies (including the 661k dataset). All 2.4 million have been uniformly reprocessed for quality criteria and to give taxonomic abundance estimates with respect to the GTDB phylogeny. We also provide antimicrobial resistance (AMR) gene and mutation annotation via AMRFinderPlus. Using an evolution-informed compression approach, the full set of genomes is just 130Gb in batched xz archives. We also provide multiple search indexes and a method for alignment to the full dataset. Finally, we outline plans for future annotations to be provided in further releases.