SIRT3
NAD+激酶
烟酰胺单核苷酸
烟酰胺腺嘌呤二核苷酸
屋尘螨
化学
癌症研究
免疫学
生物
生物化学
锡尔图因
酶
过敏
过敏原
作者
Jiayuan Liang,Chunxiang Zhou,Changyun Zhang,Shixiu Liang,Zili Zhou,Zicong Zhou,Chao Wu,Haijin Zhao,Xiang‐Jin Meng,Fei Zou,Shaoxi Cai,Shaoxi Cai
标识
DOI:10.1016/j.intimp.2023.111328
摘要
Nicotinamide adenine dinucleotide (NAD+) is an essential element in cellular metabolism that regulates fundamental biological processes. Growing evidence suggests that a decline in NAD+ is a common pathological factor in various diseases and aging. However, its role in airway epithelial barrier function in response to asthma remains underexplored. The current study aims to explore the efficacy of restoring cellular NAD+ concentration through supplementation with the NAD+ precursor, nicotinamide mononucleotide (NMN), in the treatment of allergic asthma and to investigate the role of SIRT3 in mediating the effects of NAD+ precursors. In this research, NMN alleviated airway inflammation and reduced mucus secretion in house dust mite (HDM)-induced asthmatic mice. It also mitigated airway epithelial barrier disruption in HDM-induced asthma in vitro and in vivo. But inhibition of SIRT3 expression abolished the effects of NMN. Mechanistically, HDM induced SIRT3 SUMOylation and proteasomal degradation. Mutation of these two SIRT3 SUMO modification sites enhanced the stability of SIRT3. Additionally, SIRT3 was targeted by SENP1 which acted to de-conjugate SUMO. And down-regulation of SENP1 expression in HDM-induced models was reversed by NMN. Collectively, these findings suggest that NMN attenuates airway epithelial barrier dysfunction via inhibiting SIRT3 SUMOylation in asthma. Blockage of SIRT3 SUMOylation emerges as for the treatment of allergic asthma.
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