清脆的
核糖核酸
化学
引导RNA
G-四倍体
计算生物学
分子生物学
生物
DNA
遗传学
Cas9
基因
作者
Tao Li,Dongjuan Chen,Xiaoling He,Zheyu Li,Zhichen Xu,R.Y. Li,Bingxin Zheng,Rui Hu,Jiang Zhu,Ying Li,Yunhuang Yang
摘要
In this study, we investigated Cas13a's efficacy in trans-cleaving RNA G-quadruplexes (rG4s) as an alternative to ssRNA reporters in CRISPR-Cas13a diagnostics. Our findings demonstrate enhanced efficiency due to the structural arrangement of rG4s. Implementing a simplified CRISPR-Cas13a system based on rG4, we identified SARS-CoV-2 infections in 25 patient samples within 1 hour without target pre-amplification.
科研通智能强力驱动
Strongly Powered by AbleSci AI