结垢
过滤(数学)
扫描电子显微镜
膜
膜污染
错流过滤
纤维
生物反应器
化学工程
化学
中空纤维膜
中国仓鼠卵巢细胞
色谱法
分析化学(期刊)
材料科学
复合材料
生物化学
工程类
受体
有机化学
统计
数学
作者
V. Sundar,Da Zhang,Xianghong Qian,Ranil Wickramasinghe,John Paul Smelko,Christina Carbrello,Yara Jabbour Al Maalouf,Andrew L. Zydney
摘要
Alternating tangential flow filtration (ATF) has become one of the primary methods for cell retention and clarification in perfusion bioreactors. However, membrane fouling can cause product sieving losses that limit the performance of these systems. This study used scanning electron microscopy and energy dispersive X-ray spectroscopy to identify the nature and location of foulants on 0.2 μm polyethersulfone hollow fiber membranes after use in industrial Chinese hamster ovary cell perfusion bioreactors for monoclonal antibody production. Membrane fouling was dominated by proteinaceous material, primarily host cell proteins along with some monoclonal antibody. Fouling occurred primarily on the lumen surface with much less protein trapped within the depth of the fiber. Protein deposition was also most pronounced near the inlet/exit of the hollow fibers, which are the regions with the greatest flux (and transmembrane pressure) during the cyclical operation of the ATF. These results provide important insights into the underlying phenomena governing the fouling behavior of ATF systems for continuous bioprocessing.
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