免疫系统
抗原
抗原呈递
卵清蛋白
佐剂
交叉展示
细胞生物学
淋巴结
材料科学
脾脏
内化
抗原提呈细胞
树突状细胞
CD8型
生物
T细胞
癌症研究
免疫学
细胞
遗传学
作者
Zhenyu Wang,Tianyan You,Chengyuan Cai,Qianyi Su,Jinmei Cheng,Jiaying Xiao,Xiaopin Duan
标识
DOI:10.1021/acsami.2c21028
摘要
The internalization of antigens by dendritic cells (DCs) is the initial critical step for vaccines to activate the immune response; however, the systemic delivery of antigens into DCs is hampered by various technical challenges. Here we show that a virus-like gold nanostructure (AuNV) can effectively bind to and be internalized by DCs due to its biomimetic topological morphology, thereby significantly promoting the maturation of DCs and the cross-presentation of the model antigen ovalbumin (OVA). In vivo experiments demonstrate that AuNV efficiently delivers OVA to draining lymph nodes and significantly inhibits the growth of MC38-OVA tumors, generating a ∼80% decrease in tumor volume. Mechanistic studies reveal that the AuNV-OVA vaccine induces a remarkable increase in the rate of maturation of DCs, OVA presentation, and CD4+ and CD8+ T lymphocyte populations in both lymph node and tumor and an obvious decrease in myeloid-derived suppressor cells and regulatory T cell populations in spleen. The good biocompatibility, strong adjuvant activity, enhanced uptake of DCs, and improved T cell activation make AuNV a promising antigen delivery platform for vaccine development.
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