核酸
寡核苷酸
核酶
叠氮化物
碱基
核糖核酸
连接器
DNA
RNA剪接
组合化学
生物化学
核苷
化学
生物
有机化学
基因
操作系统
计算机科学
作者
Tiziano G Bassi,Anastassia Hirlinger,Leah Grayson,Julien C. Vantourout,Navtej Toor
出处
期刊:RNA
日期:2023-06-05
卷期号:29 (9): 1437-1451
标识
DOI:10.1261/rna.079679.123
摘要
We have devised a single pot, low-cost method to add azide groups to unmodified nucleic acids without the need for enzymes or chemically modified nucleoside triphosphates. This involves reacting an azide-containing sulfinate salt with the nucleic acid, leading to replacement of C-H bonds on the nucleobase aromatic rings with C-R, where R is the azide-containing linker derived from the original sulfinate salt. With the addition of azide functional groups, the modified nucleic acid can easily be reacted with any alkyne-labeled compound of interest, including fluorescent dyes as shown in this work. This methodology enables the fluorescent labeling of a wide variety of nucleic acids, including natively folded RNAs, under mild conditions with minimal effects upon biochemical function and ribozyme catalysis. To demonstrate this, we show that a pair of labeled complementary ssDNA oligonucleotides (oligos) can hybridize to form dsDNA, even when labeled with multiple fluorophores per oligo. In addition, we also demonstrate that two different group II introns can splice when prelabeled internally with fluorophores, using our method. Broadly, this demonstrates that sulfinate modification of RNA is compatible with ribozyme function and Watson-Crick pairing, while preserving the labile backbone.
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