生物加工
细胞外基质
皮肤当量
生物医学工程
材料科学
体内
组织工程
体外
基质(化学分析)
细胞生物学
化学
医学
生物
角质形成细胞
复合材料
生物技术
生物化学
作者
Byoung Yoon Kim,Yang Woo Kwon,Jeong Sik Kong,Gyu Hwan Park,Ge Gao,Wonil Han,Moon-Bum Kim,Hyungseok Lee,Jae Nyoung Kim,Dong-Woo Cho
出处
期刊:Biomaterials
[Elsevier]
日期:2018-06-01
卷期号:168: 38-53
被引量:294
标识
DOI:10.1016/j.biomaterials.2018.03.040
摘要
3D cell-printing technique has been under spotlight as an appealing biofabrication platform due to its ability to precisely pattern living cells in pre-defined spatial locations. In skin tissue engineering, a major remaining challenge is to seek for a suitable source of bioink capable of supporting and stimulating printed cells for tissue development. However, current bioinks for skin printing rely on homogeneous biomaterials, which has several shortcomings such as insufficient mechanical properties and recapitulation of microenvironment. In this study, we investigated the capability of skin-derived extracellular matrix (S-dECM) bioink for 3D cell printing-based skin tissue engineering. S-dECM was for the first time formulated as a printable material and retained the major ECM compositions of skin as well as favorable growth factors and cytokines. This bioink was used to print a full thickness 3D human skin model. The matured 3D cell-printed skin tissue using S-dECM bioink was stabilized with minimal shrinkage, whereas the collagen-based skin tissue was significantly contracted during in vitro tissue culture. This physical stabilization and the tissue-specific microenvironment from our bioink improved epidermal organization, dermal ECM secretion, and barrier function. We further used this bioink to print 3D pre-vascularized skin patch able to promote in vivo wound healing. In vivo results revealed that endothelial progenitor cells (EPCs)-laden 3D-printed skin patch together with adipose-derived stem cells (ASCs) accelerates wound closure, re-epithelization, and neovascularization as well as blood flow. We envision that the results of this paper can provide an insightful step towards the next generation source for bioink manufacturing.
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