表面等离子共振
共振(粒子物理)
局域表面等离子体子
折射率
表面等离子体子
等离子体子
材料科学
分子
分析物
化学
配体(生物化学)
纳米技术
光学
光电子学
物理
纳米颗粒
原子物理学
物理化学
生物化学
受体
有机化学
作者
Dennis G. Drescher,Dakshnamurthy Selvakumar,Marian J. Drescher
出处
期刊:Advances in protein chemistry and structural biology
日期:2018-01-01
卷期号:: 1-30
被引量:49
标识
DOI:10.1016/bs.apcsb.2017.07.003
摘要
Surface plasmon resonance is an optical technique that is utilized for detecting molecular interactions, such as interactions that occur between proteins or other classes of molecules. Binding of a mobile molecule (analyte) to a molecule immobilized on a thin metal film (ligand) changes the refractive index of the film. The angle of extinction of light that is completely reflected after polarized light impinges upon the film, is altered and monitored as a change in detector position for a dip in reflected intensity (the surface plasmon resonance phenomenon). Because the method strictly detects mass, there is no need to label the interacting components, thus eliminating possible changes of their molecular properties. In this chapter, we review essential SPR methodology and present applications to basic science and human disease.
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