癫痫持续状态
红藻氨酸受体
脑电图
麻醉
癫痫
医学
内科学
生物
神经科学
受体
NMDA受体
AMPA受体
作者
Karen Tse,Sreekanth Puttachary,Edward Beamer,Graeme J. Sills,Thimmasettappa Thippeswamy
出处
期刊:PLOS ONE
[Public Library of Science]
日期:2014-05-06
卷期号:9 (5): e96622-e96622
被引量:73
标识
DOI:10.1371/journal.pone.0096622
摘要
A refined kainate (KA) C57BL/6J mouse model of status epilepticus (SE) using a repeated low dose (RLD) of KA (5 mg/kg, intraperitoneal; at 30 min intervals) was compared with the established single high dose (SHD) of KA (20 mg/kg, intraperitoneal) model. In the RLD group, increased duration of convulsive motor seizures (CMS, Racine scale stage ≥3) with a significant reduction in mortality from 21% to 6% and decreased variability in seizure severity between animals/batches were observed when compared to the SHD group. There was a significant increase in the percentage of animals that reached stage-5 seizures (65% versus 96%) in the RLD group. Integrated real-time video-EEG analysis of both groups, using NeuroScore software, revealed stage-specific spikes and power spectral density characteristics. When the seizures progressed from non-convulsive seizures (NCS, stage 1-2) to CMS (stage 3-5), the delta power decreased which was followed by an increase in gamma and beta power. A transient increase in alpha and sigma power marked the transition from NCS to CMS with characteristic 'high frequency trigger' spikes on the EEG, which had no behavioral expression. During SE the spike rate was higher in the RLD group than in the SHD group. Overall these results confirm that RLD of KA is a more robust and consistent mouse model of SE than the SHD of KA mouse model.
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