信使核糖核酸
生物
鸟苷
五素帽
翻译(生物学)
核糖核酸
生物化学
细胞生物学
二聚体
食腐动物
水解
酶
核苷酸
鸟嘌呤
生物物理学
化学
基因
激进的
RNA编辑
有机化学
作者
Meigang Gu,Carme Fàbrega,Shin-Wu Liu,Hudan Liu,Megerditch Kiledjian,Christopher D. Lima
出处
期刊:Molecular Cell
[Elsevier]
日期:2004-04-01
卷期号:14 (1): 67-80
被引量:119
标识
DOI:10.1016/s1097-2765(04)00180-7
摘要
Complete removal of residual N-7 guanine cap from degraded messenger RNA is necessary to prevent accumulation of intermediates that might interfere with RNA processing, export, and translation. The human scavenger decapping enzyme, DcpS, catalyzes residual cap hydrolysis following mRNA degradation, releasing N-7 methyl guanosine monophosphate and 5'-diphosphate terminated cap or mRNA products. DcpS structures bound to m(7)GpppG or m(7)GpppA reveal an asymmetric DcpS dimer that simultaneously creates an open nonproductive DcpS-cap complex and a closed productive DcpS-cap complex that alternate via 30 A domain movements. Structural and biochemical analysis suggests an autoregulatory mechanism whereby premature decapping mRNA is prevented by blocking the conformational changes that are required to form a closed productive active site capable of cap hydrolysis.
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