肝细胞
并行传输
生物物理学
化学
单层
膜
细胞培养
细胞内
细胞生物学
细胞
生物化学
生物
体外
磁导率
遗传学
作者
James L. Boyer,J. M. Phillips,J. Graf
出处
期刊:Methods in Enzymology
日期:1990-01-01
卷期号:: 501-516
被引量:40
标识
DOI:10.1016/0076-6879(90)92090-z
摘要
This chapter discusses the preparation and specific applications of isolated hepatocyte couplets. A number of different methods are available for isolating hepatocytes in suspension either for short-term experiments or as preliminary steps in the preparation of hepatocyte monolayer culture systems. Hepatocyte couplets, which are plated at lower cell density then in monolayer preparations, represent a primary secretory unit that enables direct observations of the process of canalicular bile formation at its site of origin. This cell system thus permits the application of a variety of techniques and methodologies available for studying cell function in tissue culture, including phase and Nomarski microscopy and cinephotomicrogaphy as well as fluorescent and electrophysiologic applications. Specific applications include (1) determination of transepithelial potential profile, (2) determination of the resistive elements of ionic current flow (basolateral and lumenal membrane, paracellular pathway), and (3) determination of partial ionic conductances of the basolateral cell membrane.
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