纳米探针
细胞内
化学
分子信标
癌细胞
细胞生物学
信使核糖核酸
分子生物学
生物物理学
纳米技术
纳米颗粒
癌症
生物化学
DNA
生物
基因
遗传学
材料科学
寡核苷酸
作者
Wei Pan,Tingting Zhang,Huijun Yang,Wei Diao,Na Li,Bo Tang
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2013-10-18
卷期号:85 (21): 10581-10588
被引量:190
摘要
Simultaneous detection and imaging of multiple intracellular biomarkers hold great promise for early cancer detection. Here, we introduce a four-color nanoprobe that can simultaneously detect and image four types of mRNAs in living cells. The nanoprobe composed of gold nanoparticles functionalized with a dense shell of molecular beacons, which can identify multiple intracellular mRNA transcripts. It shows rapid response, high specificity, nuclease stability, and good biocompatibility. Intracellular experiments indicate that the nanoprobe could effectively distinguish cancer cells from their normal cells, even some mRNAs are overexpressed in normal cells. Moreover, it can identify the changes of the expression levels of mRNA in living cells. The current strategy could provide more-accurate information for early cancer detection and availably avoid false positive results.
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