Transmissibility is the main performance evaluation index of Metal Rubber (MR) isolator, which can be got by sine sweep frequency test. At different temperature, the sine sweep frequency test is done with different structural parameter MR isolator. The influence that relative density and pre-deformation have on transmissibility and natural frequency when temperature changed is analyzed. The changing regularity of MR isolator’s transmissibility at different temperature is explored. Research results provide the basis for designing MR isolator.