免疫系统
细胞毒性T细胞
抗氧化剂
脂质过氧化
谷胱甘肽
乙酰半胱氨酸
低密度脂蛋白
外周血单个核细胞
化学
细胞因子
脂蛋白
T细胞
药理学
生物化学
生物
内分泌学
体外
免疫学
胆固醇
酶
作者
Marina Viora,Maria Giovanna Quaranta,Elisabetta Straface,Rosaria Varı́,Roberta Masella,Walter Malorni
出处
期刊:Immunology
[Wiley]
日期:2001-12-01
卷期号:104 (4): 431-438
被引量:34
标识
DOI:10.1046/j.1365-2567.2001.01334.x
摘要
This study investigates the in vitro effects of oxidized low-density lipoproteins (ox-LDL), 'physiological' pro-oxidants, N-acetylcysteine (NAC), a free radical scavenger and glutathione precursor, and their combination on human peripheral blood mononuclear cell functions. We found that treatment with ox-LDL induced a significant down-regulation of proliferative response to mitogens, antigens and interleukin-2. Lipid extracts from ox-LDL were able to reproduce the same effect as the lipoprotein. On the other hand, NAC exposure induced a significant up-regulation of proliferative responses to all the stimuli used. Moreover, we showed that natural killer (NK) cell-mediated cytotoxic activity was significantly down-regulated by ox-LDL while treatment with NAC induced a significant up-regulation of NK-cell activity. Finally, we found that ox-LDL and NAC exerted opposite effects on the cytokine network, interfering both at the protein secretion level and the messenger RNA synthesis level. More importantly, when NAC was used in combination with ox-LDL the proliferative responses, NK-cell-mediated cytotoxic activity and cytokine production were restored to values comparable to controls. These data indicate that ox-LDL and NAC modulate immune functions, exerting opposite effects reflecting their pro-oxidant and antioxidant behaviours. Our results add new insights to the key role played by redox imbalance as a modulator of immune system homeostasis and suggest that an antioxidant drug such as NAC could be useful against pathologies associated with an increase in lipid peroxidation.
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