果胶
橙色(颜色)
化学
葡聚糖
色谱法
铵
食品科学
生物化学
酶
有机化学
出处
期刊:Phytochemistry
[Elsevier]
日期:1991-01-01
卷期号:30 (2): 445-449
被引量:41
标识
DOI:10.1016/0031-9422(91)83701-l
摘要
Pectin acetylesterase was purified from orange peel using ammonium sulphate fractionation, followed by chromatography on Sephadex G-25 superfine, mono S and Superose 6. The purified enzyme has a Mr of 29 000. It has no pectin methylesterase activity, but rapidly de-esterifies triacetin. The pectin acetylesterase activity is increased 4.8-fold by prior incubation of sugarbeet pectin with pectin methylesterase, which suggests that the acetylesterase preferentially deacetylates galacturonyl residues containing a free C-6 carboxy group.
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