表位
细胞毒性T细胞
埃利斯波特
抗原
生物
分子生物学
人类白细胞抗原
CTL公司*
癌症免疫疗法
肿瘤抗原
癌症研究
免疫学
免疫疗法
免疫系统
体外
CD8型
生物化学
作者
Wei Liu,Mingxia Zhai,Zongyin Wu,Yuanming Qi,Yahong Wu,Chao Dai,Meng Sun,Lü Li,Yanfeng Gao
出处
期刊:Amino Acids
[Springer Nature]
日期:2011-06-28
卷期号:42 (6): 2257-2265
被引量:25
标识
DOI:10.1007/s00726-011-0966-3
摘要
Identification of cytotoxic T lymphocyte (CTL) epitopes from tumor antigens is essential for the development of peptide vaccines against tumor immunotherapy. Among all the tumor antigens, the caner-testis (CT) antigens are the most widely studied and promising targets. PLAC1 (placenta-specific 1, CT92) was considered as a novel member of caner-testis antigen, which expressed in a wide range of human malignancies, most frequently in breast cancer. In this study, three native peptides and their analogues derived from PLAC1 were predicted by T cell epitope prediction programs including SYFPEITHI, BIMAS and NetCTL 1.2. Binding affinity and stability assays in T2 cells showed that two native peptides, p28 and p31, and their analogues (p28-1Y9 V, p31-1Y2L) had more potent binding activity towards HLA-A*0201 molecule. In ELISPOT assay, the CTLs induced by these four peptides could release IFN-γ. The CTLs induced by these four peptides from the peripheral blood mononuclear cells (PBMCs) of HLA-A*02+ healthy donor could lyse MCF-7 breast cancer cells (HLA-A*0201+, PLAC1+) in vitro. When immunized in HLA-A2.1/Kb transgenic mice, the peptide p28 could induce the most potent peptide-specific CTLs among these peptides. Therefore, our results indicated that the peptide p28 (VLCSIDWFM) could serve as a novel candidate epitope for the development of peptide vaccines against PLAC1-positive breast cancer.
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