Applications of silica supports in affinity chromatography

Abstract The combined use of silica‐based chromatographic supports with immobilized affinity ligands can be used in many preparative and analytical applications. One example is the use of silica‐based affinity columns in HPLC, giving rise to a method known as high‐performance affinity chromatography (HPAC). This review discusses the role that silica has played in the development of affinity chromatography and HPAC and the applications of silica in these methods. This includes a discussion of the types of ligands that have been employed with silica and the methods by which these ligands have been immobilized. Various formats have also been presented for the use of silica in affinity chromatographic methods, including assays involving direct or indirect analyte detection, on‐line or off‐line affinity extraction, and chiral separations. The use of silica‐based affinity columns in studies of biological systems based on zonal elution and frontal analysis methods will also be considered.