秀丽隐杆线虫
转录组
生物
基因
氨基甲酸酯
异型生物质的
氨基甲酸乙酯
基因表达
遗传学
酶
生物化学
食品科学
葡萄酒
作者
Jordan J Comfort,Samantha C Chomyshen,Brandon M Waddell,Hadi Tabarraei,Cheng-Wei Wu
标识
DOI:10.1016/j.toxrep.2022.03.029
摘要
Ethyl carbamate is a common contaminant prevalent in fermented food with probable carcinogenic effects in animals. To date, other toxicological properties of ethyl carbamate are not well characterized. Using the genetic model Caenorhabditis elegans, we found that chronic exposure to ethyl carbamate during larval development impedes growth while exposure during adulthood inhibits reproduction, shortens lifespan, and promotes degeneration to dopaminergic neurons. Through whole-transcriptome RNA-sequencing, we found that ethyl carbamate invokes a widespread transcriptomic response inducing the differential expression of > 4,000 genes by at least 2-fold. Functional analysis of RNA-sequencing data revealed that up-regulated genes enrich to various neuron regulatory processes and xenobiotic defense. Gene expression analysis confirms that various genes encoding antioxidant enzymes and those functioning within phase I and II detoxification responses along with ABC transporters are highly up-regulated after ethyl carbamate exposure, suggesting the onset of oxidative stress. Overall, these findings report new toxicological properties of chronic ethyl carbamate exposure and provide new insights on its effects on transcriptome regulation in the C. elegans model.
科研通智能强力驱动
Strongly Powered by AbleSci AI