糖基化
苏氨酸
糖蛋白
丝氨酸
聚糖
生物化学
化学
生物
计算生物学
酶
作者
Jiajia Li,Bo Guo,Wenqi Zhang,Shuang Yue,Shan Huang,Song Gao,Junfeng Ma,John F. Cipollo,Shuang Yang
出处
期刊:Proteomics
[Wiley]
日期:2022-09-18
卷期号:22 (23-24): 2200156-2200156
被引量:4
标识
DOI:10.1002/pmic.202200156
摘要
O-Glycosylation is one of the most common protein post-translational modifications (PTM) and plays an essential role in the pathophysiology of diseases. However, the complexity of O-glycosylation and the lack of specific enzymes for the processing of O-glycans and their O-glycopeptides make O-glycosylation analysis challenging. Recently, research on O-glycosylation has received attention owing to technological innovation and emerging O-glycoproteases. Several serine/threonine endoproteases have been found to specifically cleave O-glycosylated serine or threonine, allowing for the systematic analysis of O-glycoproteins. In this review, we first assessed the field of protein O-glycosylation over the past decade and used bibliometric analysis to identify keywords and emerging trends. We then summarized recent advances in O-glycosylation, covering several aspects: O-glycan release, site-specific elucidation of intact O-glycopeptides, identification of O-glycosites, characterization of different O-glycoproteases, mass spectrometry (MS) fragmentation methods for site-specific O-glycosylation assignment, and O-glycosylation data analysis. Finally, the role of O-glycosylation in health and disease was discussed.
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