抗坏血酸
介电谱
检出限
循环伏安法
电化学气体传感器
核化学
电化学
化学
生物传感器
无机化学
材料科学
电极
色谱法
生物化学
食品科学
物理化学
作者
Shalini Nagabooshanam,Bhupesh Kumar Lad,Sharmiladevi Ramamoorthy,Nishakavya Saravanan,Anandhakumar Sundaramurthy
出处
期刊:Chemosphere
[Elsevier]
日期:2024-01-01
卷期号:346: 140517-140517
标识
DOI:10.1016/j.chemosphere.2023.140517
摘要
We report a facile one-pot synthesis of bimetallic nickel-gold (Ni–Au) nanocomposite for ultra-sensitive and selective electrochemical detection of oxidized glutathione (GSSG) by electrochemical deposition on fluorine doped tin oxide (FTO) substrate. The electrodeposition of Ni–Au nanocomposite on FTO was confirmed by various characterization techniques such as field emission scanning electron microscopy (FE-SEM), X-ray diffractometer (XRD) and Fourier transform infra-red (FTIR) spectroscopy. The cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) was utilized for the electrochemical characterization of glutathione reductase (GR)/Ni–Au/FTO working electrode at each stage of modification. The GR enzyme immobilized on the Ni–Au/FTO working electrode via glutaraldehyde cross-linking exhibited excellent selectivity against GSSG in the presence of nicotinamide adenine dinucleotide phosphate (NADPH). The immobilized GR enzyme breaks down the GSSG to reduced glutathione (GSH) and converting NADPH to NADP+ whereby generating an electron for the electrochemical sensing of GSSG. The synergistic behavior of bimetals and good electro-catalytic property of the fabricated sensor provided a broad linear detection range from 1 fM to 1 μM with a limit of detection (LOD) of 6.8 fM, limit of quantification (LOQ) of 20.41 fM and sensitivity of 0.024 mA/μM/cm2. The interference with other molecules such as dopamine, glycine, ascorbic acid, uric acid and glucose was found to be negligible due to the better selectivity of GR enzyme towards GSSG. The shelf-life and response time of the fabricated electrode was found to be 30 days and 32 s, respectively. The real sample analysis of GSSG in whole blood samples showed average recovery percentage from 95 to 101% which matched well with the standard calibration plot of the fabricated sensor with %relative standard deviation (RSD) below 10%.
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