Identification of Acid Red 73 (CI 27290) in Cosmetic Hair Dye Preparations by High-Performance Liquid Chromatography – Photo Diode Array

Abstract Acid Red 73 (CI 27290) is a prohibited component in cosmetics, particularly in hair color formulations. The purpose of this investigation is to discover the coloring additive Acid Red 73 in cosmetic hair dye formulations. Acid Red 73 (CI 27290) is a sulfonated azo dye that is manufactured to be more hazardous than other colors and is damaging to the body. Based on variations in polarity and solubility, Acid Red 73 (CI 27290) is separated from the sample matrix and identified using High Performance Liquid Chromatography-Photo Diode Array (HPLC-PDA). The findings obtained from the tested samples satisfied the criteria since they did not include Acid Red 73 (CI 27290), as indicated by the difference in retention time and wavelength between the sample, the standard solution, and the spiked sample solution on the chromatogram.