Interaction of species A rotavirus VP4 with the cellular proteins vimentin and actin related protein 2 discovered by a proximity interactome assay

生物 波形蛋白 相互作用体 内化 衣壳 杯状病毒科 轮状病毒 肌动蛋白细胞骨架 病毒学 病毒进入 细胞生物学 病毒 病毒复制 细胞骨架 细胞 基因 免疫学 遗传学 免疫组织化学 病毒性疾病
作者
Pengfei Hao,Qiaoqiao Qu,Zhaoxia Pang,Letian Li,Shouwen Du,Limin Shang,Chaozhi Jin,Xu Wang,Zhuo Ha,Yuhang Jiang,Jing Chen,Zihan Gao,Ningyi Jin,Jian Wang,Chang Li
出处
期刊:Journal of Virology [American Society for Microbiology]
卷期号:97 (12) 被引量:1
标识
DOI:10.1128/jvi.01376-23
摘要

ABSTRACT Rotavirus (RV) is one of the most significant pathogens in humans and animals with diarrhea worldwide. Cell entry is the first step in viral infection, and the outer capsid protein VP4 is crucial for RV attachment and internalization. In order to discover novel candidate host factors involved in RV cell entry, a proximity labeling method was applied to systematically investigate the VP4 and host protein interactions. A total of 174 high-confidence host proteins were identified using proximity labeling. Further analysis showed that 88 proteins were located in the cytoskeleton, plasma membrane, and extracellular region, which could be involved in RV entry. Importantly, vimentin (VIM) and actin-related protein 2 (ACTR2) were identified to promote RV infection at an early step. The results of co-immunoprecipitation assay showed that VIM or ACTR2 physically interacted with VP4. Blocking VIM or ACTR2 function by silencing with small interfering RNA or inhibition by specific antibodies significantly restricted RV infection. Furthermore, increasing the amounts of VIM or ACTR2 by overexpression from transfected recombinant proteins or incubation with recombinant proteins promoted RV infection. Collectively, this study revealed that RV VP4 interacted with host proteins and demonstrated that interaction with VIM and ACTR2 promoted RV replication, providing valuable resources and potential drug targets for better understanding and treating this disease. IMPORTANCE Rotavirus (RV) is an important zoonosis virus, which can cause severe diarrhea and extra-intestinal infection. To date, some proteins or carbohydrates have been shown to participate in the attachment or internalization of RV, including HGBAs, Hsc70, and integrins. This study attempted to indicate whether there were other proteins that would participate in the entry of RV; thus, the RV VP4-interacting proteins were identified by proximity labeling. After analysis and verification, it was found that VIM and ACTR2 could significantly promote the proliferation of RV in intestinal cells. Through further viral binding assays after knockdown, antibody blocking, and recombinant protein overexpression, it was revealed that both VIM and ACTR2 could promote RV replication.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
luonayi发布了新的文献求助10
2秒前
3秒前
秋雪瑶应助多多采纳,获得10
3秒前
清脆糖豆发布了新的文献求助10
4秒前
Fishpieee发布了新的文献求助10
5秒前
7秒前
汪哈七发布了新的文献求助10
8秒前
gjww应助机灵蛋挞采纳,获得10
8秒前
8秒前
luonayi完成签到,获得积分10
8秒前
烟花应助wuyu采纳,获得10
9秒前
abet完成签到,获得积分10
10秒前
zd完成签到,获得积分10
10秒前
单于笑卉发布了新的文献求助10
13秒前
17秒前
21秒前
清脆糖豆发布了新的文献求助10
21秒前
21秒前
栀子关注了科研通微信公众号
25秒前
姚老表完成签到,获得积分10
25秒前
华贞发布了新的文献求助10
25秒前
汪哈七完成签到,获得积分10
27秒前
27秒前
A1234567发布了新的文献求助10
28秒前
30秒前
31秒前
奥里给完成签到 ,获得积分10
31秒前
搜集达人应助Liuyan采纳,获得30
32秒前
江湖笑发布了新的文献求助10
34秒前
34秒前
35秒前
35秒前
36秒前
36秒前
婷123发布了新的文献求助10
38秒前
39秒前
彩色冥幽发布了新的文献求助10
39秒前
贪玩元晴发布了新的文献求助10
39秒前
李爱国应助zjh采纳,获得10
40秒前
丸子发布了新的文献求助10
40秒前
高分求助中
Teaching Social and Emotional Learning in Physical Education 900
Plesiosaur extinction cycles; events that mark the beginning, middle and end of the Cretaceous 800
Recherches Ethnographiques sue les Yao dans la Chine du Sud 500
Two-sample Mendelian randomization analysis reveals causal relationships between blood lipids and venous thromboembolism 500
Chinese-English Translation Lexicon Version 3.0 500
[Lambert-Eaton syndrome without calcium channel autoantibodies] 460
Wisdom, Gods and Literature Studies in Assyriology in Honour of W. G. Lambert 400
热门求助领域 (近24小时)
化学 材料科学 医学 生物 有机化学 工程类 生物化学 纳米技术 物理 内科学 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 电极 光电子学 量子力学
热门帖子
关注 科研通微信公众号,转发送积分 2393830
求助须知:如何正确求助?哪些是违规求助? 2097779
关于积分的说明 5286026
捐赠科研通 1825262
什么是DOI,文献DOI怎么找? 910154
版权声明 559943
科研通“疑难数据库(出版商)”最低求助积分说明 486418