生物
核糖核酸
感应(电子)
芜菁花叶病毒
RNA病毒
病毒复制
病毒学
植物病毒
RNA依赖性RNA聚合酶
RNA沉默
病毒
遗传学
基因
马铃薯Y病毒
RNA干扰
电气工程
工程类
作者
Pan Gong,Qingtang Shen,Mingzhen Zhang,Rui Qiao,Jing Jiang,Lili Song,Shuaiyang Zhao,Shuai Fu,Rui Zhang,Linhao Ge,Yaqin Wang,Rosa Lozano‐Durán,Aiming Wang,Fangfang Li,Xueping Zhou
出处
期刊:Molecular Plant
[Elsevier]
日期:2023-11-01
卷期号:16 (11): 1794-1810
被引量:5
标识
DOI:10.1016/j.molp.2023.09.020
摘要
Positive-sense single-stranded RNA (+ssRNA) viruses, the most abundant viruses of eukaryotes in nature, require the synthesis of negative-sense RNA (-RNA) using their genomic (positive-sense) RNA (+RNA) as a template for replication. Based on current evidence, viral proteins are translated via viral +RNAs, whereas -RNA is considered to be a viral replication intermediate without coding capacity. Here, we report that plant and animal +ssRNA viruses contain small open reading frames (ORFs) in their -RNA (reverse ORFs [rORFs]). Using turnip mosaic virus (TuMV) as a model for plant +ssRNA viruses, we demonstrate that small proteins encoded by rORFs display specific subcellular localizations, and confirm the presence of rORF2 in infected cells through mass spectrometry analysis. The protein encoded by TuMV rORF2 forms punctuate granules that are localized in the perinuclear region and co-localized with viral replication complexes. The rORF2 protein can directly interact with the viral RNA-dependent RNA polymerase, and mutation of rORF2 completely abolishes virus infection, whereas ectopic expression of rORF2 rescues the mutant virus. Furthermore, we show that several rORFs in the -RNA of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have the ability to suppress type I interferon production and facilitate the infection of vesicular stomatitis virus. In addition, we provide evidence that TuMV might utilize internal ribosome entry sites to translate these small rORFs. Taken together, these findings indicate that the -RNA of +ssRNA viruses can also have the coding capacity and that small proteins encoded therein play critical roles in viral infection, revealing a viral proteome larger than previously thought.
科研通智能强力驱动
Strongly Powered by AbleSci AI