Tissue culture of Juniperus chinensis : effects of sex and leaf morphology on in vitro regeneration

This study established a multi-dimensional tissue culture system for J. chinensis based on three sexes (FP, MP and MOP) and their corresponding SL and SPL. It analysed the differences and preferences among different sexes and leaf forms at various stages of tissue culture, providing technical support for the targeted breeding, germplasm resource innovation and industrial development of J. chinensis. Meanwhile, it offers novel perspectives for research on sex differentiation, mechanisms of leaf form transformation and other related areas in polygamous or heterophyllous tree species.