生物
基因
转录组
突变体
转录因子
蛋白激酶A
激酶
真菌
WRKY蛋白质结构域
细胞生物学
免疫系统
遗传学
拟南芥
基因表达
植物
作者
Yingying Cao,Mengfei Lu,Jinhui Chen,Wenyan Li,Mo Wang,Fengping Chen
出处
期刊:Plants
[MDPI AG]
日期:2024-02-22
卷期号:13 (5): 596-596
标识
DOI:10.3390/plants13050596
摘要
Sucrose non-fermenting–1-related protein kinase–1 (SnRK1) is a highly conserved serine–threonine kinase complex regulating plants’ energy metabolisms and resistance to various types of stresses. However, the downstream genes regulated by SnRK1 in these plant physiological processes still need to be explored. In this study, we found that the knockout of OsSnRK1a resulted in no obvious defects in rice growth but notably decreased the seed setting rate. The ossnrk1a mutants were more sensitive to blast fungus (Magnaporthe oryzae) infection and showed compromised immune responses. Transcriptome analyses revealed that SnRK1a was an important intermediate in the energy metabolism and response to biotic stress. Further investigation confirmed that the transcription levels of OsNADH-GOGAT2, which positively controls rice yield, and the defense-related gene pathogenesis-related protein 1b (OsPR1b) were remarkably decreased in the ossnrk1a mutant. Moreover, we found that OsSnRK1a directly interacted with the regulatory subunits OsSnRK1β1 and OsSnRK1β3, which responded specifically to blast fungus infection and starvation stresses, respectively. Taken together, our findings provide an insight into the mechanism of OsSnRK1a, which forms a complex with specific β subunits, contributing to rice seed set and resistance by regulating the transcription of related genes.
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