化学
DNA
检出限
流式细胞术
核糖核酸
小RNA
计算生物学
荧光
底漆(化妆品)
生物传感器
纳米技术
分子生物学
基因
色谱法
生物化学
生物
物理
材料科学
有机化学
量子力学
作者
Zhenxiang Liu,Nuli Xie,Juan Li,Xiaoxiao Fu,Kemin Wang,Hui Shi,Jin Huang
标识
DOI:10.1021/acs.analchem.2c05648
摘要
Sensitive detection of miRNA targets in complex biological samples possesses great value in biopsy analysis and disease diagnosis but is still challenging because of low abundance and nonspecific interferences. In this work, self-primer DNA polymerization-propelled stochastic walkers (SWs) were proposed to detect miRNA-24 by combining magnetic microbeads (MMBs) and flow cytometry. The MMBs not only provide a three-dimensional interface for DNA walkers but also facilitate the enrichment and isolation of RNA targets from complex biological samples such as serum. The SWs can be initiated to walk through the entire surface of MMBs and transduce RNA walking into amplified fluorescence signals, with the detection limit of miRNA-24 at 0.95 pM. Moreover, this strategy integrating with flow cytometry was demonstrated to have good specificity with other homologous miRNAs. This platform offers promising applications in RNA biosensing and biomedical diagnostics.
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