自愈水凝胶
壳聚糖
甲壳素
生物相容性
脚手架
收缩率
材料科学
生物材料
三维细胞培养
胡须
化学工程
化学
纳米技术
生物医学工程
高分子化学
复合材料
体外
生物化学
冶金
工程类
医学
作者
Yanyan Zhu,Di Qin,Jinfeng Liu,Guangsheng Wu,Haonan Wang,Feifei Wu,Yixuan Liu,Ya Liu,Xiaojie Cheng,Xiguang Chen
标识
DOI:10.1016/j.carbpol.2022.120483
摘要
Hydrogel, as three-dimensional (3D) cell culture scaffold, is an effective strategy for tissue and organ regeneration due to their good biocompatibility, biodegradability and resemblance to body microenvironments in vivo. However, the inherent weak mechanical properties and strong shrinkage of hydrogels during cell culture hinder its application in clinical. In this study, a two-component thermo/photo dual-sensitive hydrogel (M/C) was prepared from methacrylated hydroxybutyl chitosan (MHBC) and chitin whisker (CHW) via physical and chemical cross-linking methods. M/C hydrogel showed a special internal structure with lamellar arrangement. The rheological properties of the hydrogels could be regulated with the change of M/C ratio. It is worth emphasizing that the mechanical properties, shrinkage resistance and cellular capacitances of the M/C hydrogel were improved with the addition of CHW. Moreover, the M/C hydrogel not only exhibited excellent degradability and antibacterial properties, but also significantly promoted the adhesion and proliferation of MC3T3-E1 cells in vitro. Therefore, the M/C hydrogel showed a wide application potential in tissue regeneration as a 3D cell culture scaffold.
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