生物修复
Mercury(编程语言)
细菌
化学
挥发
环境化学
操纵子
假单胞菌
节杆菌
食品科学
微生物学
生物化学
生物
基因
大肠杆菌
有机化学
酶
程序设计语言
遗传学
计算机科学
作者
Junjun Chang,Zhengjian Yan,Jia Hong Dong,Xinni Wu,Zhuang Meng,Yu Shi,Jinquan Chen
标识
DOI:10.1016/j.jhazmat.2021.127948
摘要
Bioremediation using mercury (Hg)-volatilizing and immobilizing bacteria is an eco-friendly and cost-effective strategy for Hg-polluted farmland. However, the mechanisms controlling the transformation of and resistance to Hg(II) by these bacteria remain unknown. In this study, a plant-associated Pseudomonas sp. strain, AN-B15 was isolated and determined to effectively remove Hg(II) under both nutrient-poor and nutrient-rich conditions via volatilization by transforming Hg(II) to Hg(0) and immobilization by transforming Hg(II) to mercury sulfide and Hg-sulfhydryl. Genome and transcriptome analyses revealed that the molecular mechanisms involved in Hg(II) resistance in AN-B15 were a collaborative process involving multiple metabolic systems at the transcriptional level. Under Hg(II) stress, AN-B15 upregulated genes involved in the mer operon and producing the reducing power to rapidly volatilize Hg(II), thereby decreasing its toxicity. Hydroponic culture experiments also revealed that inoculation with strain AN-B15 alleviated Hg-induced toxicity and reduced the uptake of Hg(II) in the roots of wheat seedlings, as explained by the volatilization and immobilization of Hg(II) and plant growth-promoting traits of AN-B15. Overall, the results from the in vitro assays provided vital information that are essential for understanding the mechanism of Hg(II) resistance in plant-associated bacteria, which can also be applied for the bioremediation of Hg-contamination in future.
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