自噬
安普克
PI3K/AKT/mTOR通路
ULK1
细胞凋亡
骨肉瘤
癌症研究
化学
细胞生物学
磷酸化
材料科学
药理学
医学
生物化学
蛋白激酶A
生物
作者
Xiao-Gang Wei,Zhi Tang,Hao Wu,Xiaohang Zuo,Hongyan Dong,Lijie Tan,W. Wang,Y. Liu,Zhigang Wu,Lei Shi,N. Wang,X. Li,Xin Xiao,Zheng Guo
标识
DOI:10.1016/j.mtbio.2021.100147
摘要
The recurrence of osteosarcoma (OS) after reconstruction using Ti6Al4V prostheses remains a major problem in the surgical treatment of OS. Modification of the surfaces of Ti6Al4V prostheses with antitumor functions is an important strategy for improving therapeutic outcomes. Magnesium (Mg) coating has been shown to be multifunctional: it exhibits osteogenic and angiogenic properties and the potential to inhibit OS. In this study, we determined the proper concentration of released Mg2+ with respect to OS inhibition and biosafety and evaluated the anti-OS effects of Mg-coated Ti6Al4V scaffolds. We found that the release of Mg2+ during short-term and long-term degradation could significantly inhibit the proliferation and migration of HOS and 143B cells. Increased cell apoptosis and excessive autophagy were also observed, and further evidence of AMPK/mTOR/ULK1 signaling pathway activation was obtained both in vitro and in vivo, which suggested that the biofunctional scaffolds induce OS inhibition. Our study demonstrates the ability of an Mg coating to inhibit OS and may contribute to the further application of Mg-coated Ti6Al4V prostheses.
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