生物
白色念珠菌
细胞生物学
菌丝
突变体
分泌物
微生物学
细胞壁
肌动蛋白细胞骨架
形态发生
肌动蛋白
白色体
磷酸酶
酿酒酵母
毒力
真菌蛋白
细胞
细胞骨架
酵母
生物化学
基因
磷酸化
作者
Bing Zhang,Qilin Yu,Chang Jia,Yuzhou Wang,Chenpeng Xiao,Yijie Dong,Ning Xu,Lei Wang,Mingchun Li
标识
DOI:10.1016/j.fgb.2014.12.007
摘要
Candida albicans is a common pathogenic fungus and has aroused widespread attention recently. Actin cytoskeleton, an important player in polarized growth, protein secretion and organization of cell shape, displays irreplaceable role in hyphal development and cell integrity. In this study, we demonstrated a homologue of Saccharomyces cerevisiae Sac1, in C. albicans. It is a potential PIP phosphatase with Sac domain which is related to actin organization, hyphal development, biofilm formation and cell wall integrity. Deletion of SAC1 did not lead to insitiol-auxotroph phenotype in C. albicans, but this gene rescued the growth defect of S. cerevisiae sac1Δ in the insitiol-free medium. Hyphal induction further revealed the deficiency of sac1Δ/Δ in hyphal development and biofilm formation. Fluorescence observation and real time PCR (RT-PCR) analysis suggested both actin and the hyphal cell wall protein Hwp1 were overexpressed and mislocated in this mutant. Furthermore, cell wall integrity (CWI) was largely affected by deletion of SAC1, due to the hypersensitivity to cell wall stress, changed content and distribution of chitin in the mutant. As a result, the virulence of sac1Δ/Δ was seriously attenuated. Taken together, this study provides evidence that Sac1, as a potential PIP phosphatase, is essential for actin organization, hyphal development, CWI and pathogenicity in C. albicans.
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