骨桥蛋白
骨钙素
碱性磷酸酶
二硫化钼
纳米复合材料
骨形态发生蛋白2
体内
材料科学
细胞生物学
再生(生物学)
生物医学工程
体外
化学
纳米技术
生物化学
生物
免疫学
医学
酶
生物技术
冶金
作者
Umakant Yadav,Harshit Mishra,Vimal Singh,Sonu Kashyap,Anchal Srivastava,Sanjay Yadav,Preeti S. Saxena
出处
期刊:ACS Biomaterials Science & Engineering
[American Chemical Society]
日期:2019-07-17
卷期号:5 (9): 4511-4521
被引量:31
标识
DOI:10.1021/acsbiomaterials.9b00227
摘要
The advances in the arena of biomedical engineering enable us to fabricate novel biomaterials that provide a suitable platform for rapid bone regeneration. Herein, we have investigated the in vitro and in vivo osteogenic differentiation, proliferation, and bone regeneration capability of molybdenum disulfide nanosheets (MoS2NSs) reinforced HAP nanocomposite scaffolds. The MG-63 cells were incubated with HAP and HAP/MoS2NSs nanocomposite and followed for various cellular activities. The cells incubated with HAP@2 shows higher cell adhesion, cell proliferation, and alkaline phosphatase activity (ALP) in contrast to HAP. The in vivo and in vitro results of the increased ALP level confirm that HAP@2 promotes osteogenic differentiation. This improved osteogenesis was validated with upregulation of osteogenic marker viz. transcription factor, RUNX-2 (∼34 fold), collagen-1 (∼15 fold), osteopontin (∼11 fold), osteocalcin (∼20 fold), and bone morphogenetic protein-2 (∼12 fold) after 12 week postimplantation in comparison to drilled. The X-ray imaging demonstrates that HAP@2 implants promote rapid osteogenesis and bioresorbability than HAP and drilled. The outcomes of the present study provide a promising tool for the regeneration of bone deformities, without using any external growth factor.
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