淋巴结
脂肪生成
生物
脂肪组织
增强子
信使核糖核酸
核糖核酸
基因
基因表达
细胞生物学
分子生物学
遗传学
内分泌学
生态学
作者
Jieping Huang,Qiuzhi Zheng,Shuzhe Wang,Xuefeng Wei,Fen Li,Yun Ma
出处
期刊:Genes
[MDPI AG]
日期:2019-09-06
卷期号:10 (9): 689-689
被引量:15
标识
DOI:10.3390/genes10090689
摘要
The buffalo (Bubalus bubalis.L) is prevalent in China and the increasing demand for meat production has changed its role from being a beast of burden to a meat source. The low fat deposition level has become one of the main barriers for its use in meat production. It is urgent to reveal factors involved in fat deposition in buffalo. This study performed RNA sequencing to investigate both long noncoding RNAs (lncRNAs) and mRNAs of adipose tissues in young and adult buffalos. A total of 124 lncRNAs and 2008 mRNAs showed differential expression patterns between young and adult samples. Coexpression analysis and functional enrichment revealed 585 mRNA–lncRNA pairs with potential function in fat deposition. After validation by qRT-PCR, we focused on a lncRNA transcribed from the ubiquinone oxidoreductase subunit C2 (NDUFC2) antisense (AS) strand which showed high correlation with thyroid hormone responsive protein (THRSP). NDUFC2-AS lncRNA is highly expressed in adipose tissue and maturation adipocytes and mainly exists in the nucleus. Functional assays demonstrated that NDUFC2-AS lncRNA promotes adipogenic differentiation by upregulating the expression levels of THRSP and CCAAT enhancer binding protein alpha (C/EBPα) in buffalo. These results indicate that NDUFC2-AS lncRNA promotes fat deposition in buffalo.
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