Measurement of Peroxiredoxin Activity

Abstract Peroxiredoxins are cysteine‐dependent peroxidases that react with hydrogen peroxide, larger hydroperoxide substrates, and peroxynitrite. Protocols are provided to measure Prx activity with peroxide by (1) a coupled reaction with NADPH, thioredoxin reductase, and thioredoxin, (2) the direct monitoring of thioredoxin oxidation, (3) competition with horseradish peroxidase, and (4) peroxide consumption using the FOX assay. Curr. Protoc. Toxicol . 49:7.10.1‐7.10.28. © 2011 by John Wiley & Sons, Inc.