MOESM8 of Osteopontin-integrin interaction as a novel molecular target for antibody-mediated immunotherapy in adult T-cell leukemia

Additional file 8: Figure S7. OPN produced by MEFs promotes the tumor growth and metastasis of 43Tb(−) cells inoculated into NOG mice. Six-week-old female NOG mice were subcutaneously inoculated with 43Tb(−) cells alone (n = 3) or together with either WT MEFs (n = 3) or OPN KO MEFs (n = 3) (1 × 107 cells per cell type). WT-MEFs (n = 1) or OPN KO MEFs (n = 1) only were also inoculated as negative controls. (A) The tumor size was measured every 3–4 days. (B) Blood was collected from the tail vein for detection of metastatic cells as quantified by the number of tax genes assessed using qPCR. For (A) and (B), bars indicate mean values ± SEM. Statistically significant differences are shown with P value (*P < 0.05, **P < 0.01). For (A) and (B), filled square, 43Tb(−) + WT MEFs; open square, 43Tb(−) + OPN KO MEFs; filled circle, 43Tb(−) only; open circle, WT MEFs; filled triangle, OPN KO MEFs.