X射线光电子能谱
生物膜
材料科学
粒子(生态学)
铜绿假单胞菌
胶体晶体
纳米技术
粘附
胶体
化学工程
纳米
扫描电子显微镜
细菌
化学
有机化学
复合材料
生物
工程类
遗传学
生态学
作者
Hitesh Pingle,Peng-Yuan Wang,Helmut Thissen,Peter Kingshott
出处
期刊:Small
[Wiley]
日期:2018-04-01
卷期号:14 (14): 1703574-1703574
被引量:12
标识
DOI:10.1002/smll.201703574
摘要
Micro- and nanotopographies can interfere with bacteria attachment, however, the interplay existing between surface chemistry and topography remains unclear. Here, self-assembled spherical micrometer- silica and nanometer poly(methyl methacrylate) (PMMA)-sized particles are used to make binary colloidal crystal (BCC) topographical patterns to study bacterial attachment. A uniform surface chemistry of allylamine plasma polymer (AAMpp) is coated on the top of the BCCs to study only the topography effects. The uncoated and coated BCCs are exposed to Pseudomonas aeruginosa, and the surfaces and bacteria are characterized using scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and fluorescence microscopy. It is found that bacteria attachment to the uncoated BCCs is delayed and individual cells are attracted to the small particle regions of the patterns. Surprisingly, this phenomenon is also observed for the AAMpp-coated BCCs, with bacteria attaching to the small particle regions of the pattern, in stark contrast to uniform flat films of AAMpp that are highly adhesive toward P. aeruginosa. Also, the overall levels of bacterial attachment are significantly reduced by the BCC patterns compared to controls. Thus, there is a trade-off that exists between chemistry and topography that can be exploited to delay the onset of P. aeruginosa biofilm formation on surfaces.
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